WHITACRE , David Martin , 1943 - ENDOSULFAN METABOLISM IN TEMPERATURE - STRESSED RATS

This project consisted of two separate studies. The first was conducted with the two isomers of endo14 sulfan and three of its metabolites, the other with C technical endosulfan. The primary objective of the first study was to examine qualitatively and quantitatively differences in toxicant metabolism among rats subjected to low, normal, and high temperatures. Young adult, male rats were dosed orally with endosulfan I or II and placed in metabolism units designed to collect feces and urine separately. Pairs of animals with controls thus treated, were subjected o for twenty four hours, in different tests to either 15 C, 25° C or 35° C ambient temperatures while metabolizing the administered doses. At the end of each study, organs, tissues and excreta were collected from all animals, extracted, and analyzed using gas and thin layer chroma­ tography. To examine further the scheme of endosulfan metabo­ lism under temperature stress conditions, endosulfan diol, lactone and alpha-hydroxy ether were administered to rats,